Back to Test DirectoryTB34 : BRAF/EGFR NGS if EGFR neg rfx to ALK FISH if neg rfx ROS1
INFORMATION:
Alternate Name:
BRAF/EGFR rfx >ALK>ROS1
Methodology:
Genotyping by Next Generation Sequencing
Clinical Utility:
KRAS assesses for resistance to anti-EGFR therapy in metastatic colorectal cancer and TKI therapy resistance in lung cancer. BRAF V600E/K mutation analysis is useful in the context of melanoma, colorectal cancer, thyroid cancer, and hairy cell leukemia. Melanoma patients with V600E/K mutations are eligible for treatment with TKI inhibitor therapy. In colorectal cancer, BRAF may be used as a screening assay for MSI-H or unstable patients suspected of Lynch Syndrome. BRAF V600E can aid in the diagnosis of papillary thyroid cancer (PTC) from cytology samples. Numerous studies have shown BRAF to be associated with aggressive clinicopathologic features of PTC. BRAF can also be used to confirm a diagnosis of Hairy Cell Leukemia.
ORDERING:
Test Code:
TB34-4
Turnaround Time:
10 DAYS
Preferred Specimen:
Formalin-fixed, Paraffin-embedded Tissue
Collection:
| Container | Qty | Temp | Stability |
|---|---|---|---|
| Formalin-fixed, Paraffin-embedded Tissue | 1 | Room Temp | 999 DAYS |
Collection Instructions:
BLK: This comes in block form from client with surgical number imprint.
Storage Transport Instructions:
Custom Instruction: 10-15 unstained slides at 5 microns (tumor content>10%) with H&E, Shavings (if tumor content >40%) with H&E, if sending DNA - Please call CS.
BML/BMG: Place bone marrow into tube, label with patient's name and send in provided kit.
LPB/GPB: Place peripheral blood into tube, label with patient's name and send in provided kit.
Extracted DNA is acceptable, providing that the isolation of nucleic acid occurs in a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by CMS and/or the CAP. Specimen can be either RT or refrigerated. Ship with ice pack during warm weather.
BML/BMG: Place bone marrow into tube, label with patient's name and send in provided kit.
LPB/GPB: Place peripheral blood into tube, label with patient's name and send in provided kit.
Extracted DNA is acceptable, providing that the isolation of nucleic acid occurs in a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by CMS and/or the CAP. Specimen can be either RT or refrigerated. Ship with ice pack during warm weather.
Specimen Comment:
Volume requirement for BML - Bone Marrow - Lavender Top, BMDNA - Bone Marrow DNA, BMG - Bone Marrow - Green Top, GPB - Peripheral Blood - Green Top, and LPB - Peripheral Blood - Lavender Top is 2mL , Volume Requirement for DNA-Eppendorf tube is 60uL with a minimum total DNA mass of 40ng.
Alternative Specimen:
Eppendorf tube, Shavings, Unstained Slide, Bone Marrow - Lavender Top, Bone Marrow DNA, Bone Marrow - Green Top, Peripheral Blood - Green Top, Peripheral Blood - Lavender Top
Billing:
CPT Codes:
81210 x 1, 81235 x 1
CPT Code Disclaimer
CPT codes provided are for informational purposes only. Accuracy of CPT presented should be validated prior to consideration for billing.
CPT coding is the sole responsibility of the billing party. Please direct any questions regarding CPT coding to the payer being billed.
