Back to Test DirectoryJ106 : EGFR, if neg reflex to ALK, if neg reflex to ROS1
INFORMATION:
Alternate Name:
EGFR RFX >ALK>ROS1
Methodology:
Genotyping by Next Generation Sequencing
Clinical Utility:
Reflex algorithm for potential eligibility for targeted therapies in NSCLC. EGFR mutations testing.
ORDERING:
Test Code:
J106-6
Turnaround Time:
5-10 DAYS
Preferred Specimen:
Formalin-fixed, Paraffin-embedded Tissue
Collection:
| Container | Qty | Temp | Stability |
|---|---|---|---|
| Formalin-fixed, Paraffin-embedded Tissue | 1 | Room Temp | 999 DAYS |
Collection Instructions:
BLK: This comes in block form from client with surgical number imprint.
Storage Transport Instructions:
Custom Instruction: 10-15 unstained slides at 5 microns (tumor content>10%) with H&E, Shavings (if tumor content >40%) with H&E, if sending DNA - Please call CS.
BML/BMG: Place bone marrow into tube, label with patient's name and send in provided kit.
LPB/GPB: Place peripheral blood into tube, label with patient's name and send in provided kit.
Extracted DNA is acceptable, providing that the isolation of nucleic acid occurs in a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by CMS and/or the CAP. Specimen can be either RT or refrigerated. Ship with ice pack during warm weather.
BML/BMG: Place bone marrow into tube, label with patient's name and send in provided kit.
LPB/GPB: Place peripheral blood into tube, label with patient's name and send in provided kit.
Extracted DNA is acceptable, providing that the isolation of nucleic acid occurs in a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by CMS and/or the CAP. Specimen can be either RT or refrigerated. Ship with ice pack during warm weather.
Specimen Comment:
Alternative specimen: Extracted DNA is acceptable, providing that the isolation of nucleic acid occurs in a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by CMS and/or the CAP.
Volume requirement for BML - Bone Marrow - Lavender Top, BMDNA - Bone Marrow DNA, BMG - Bone Marrow - Green Top, GPB - Peripheral Blood - Green Top, and LPB - Peripheral Blood - Lavender Top is 2mL , Volume Requirement for DNA-Eppendorf tube is 60uL with a minimum total DNA mass of 40ng.
Volume requirement for BML - Bone Marrow - Lavender Top, BMDNA - Bone Marrow DNA, BMG - Bone Marrow - Green Top, GPB - Peripheral Blood - Green Top, and LPB - Peripheral Blood - Lavender Top is 2mL , Volume Requirement for DNA-Eppendorf tube is 60uL with a minimum total DNA mass of 40ng.
Alternative Specimen:
Eppendorf tube, Shavings, Unstained Slide, Bone Marrow - Lavender Top, Bone Marrow DNA, Bone Marrow - Green Top, Peripheral Blood - Green Top, Peripheral Blood - Lavender Top
Billing:
CPT Codes:
81235 x 1
CPT Code Disclaimer
CPT codes provided are for informational purposes only. Accuracy of CPT presented should be validated prior to consideration for billing.
CPT coding is the sole responsibility of the billing party. Please direct any questions regarding CPT coding to the payer being billed.
