J185 : HIV-1 Genotype

INFORMATION:

Alternate Name:

HIV Drug Resistance,HIV Genotype,HIV1 Genotype,HIV1 Protease

Methodology:

Real-Time Polymerase Chain Reaction

Clinical Utility:

This test is intended for use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV-1 infected patients. The test identifies drug resistance-associated mutations in the HIV-1 protease and reverse transcriptase genes. It can be used to predict antiretroviral drug resistance before initiation of therapy and in patients experiencing virologic failure while on therapy.

ORDERING:

Test Code:

J185-0

Turnaround Time:

13 DAYS

Preferred Specimen:

2 mL Aliquot Plasma-EDTA-Lavender Top

Collection:

Container	Qty	Temp	Stability
Aliquot Plasma-EDTA-Lavender Top	1	Freeze, Refrigerate	6 DAYS

Collection Instructions:

ALQE: Centrifuge lavender-top at high speed for 15 minutes. Transfer plasma into plastic transfer tube. Label with date/time collected,EDTA PLASMA and patient's name.

Storage Transport Instructions:

Custom Instruction: Plasma: Freshly drawn specimens (whole blood) may be stored at 2°C to 25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. Follow manufacturer’s instructions for collection tube handling. CSF: Collect CSF using standard procedures. Transfer to a sterile, leak-proof container. Ship frozen.

Specimen Comment:

Stability On Cells: Ambient: 6 hours; After separation from cells: Ambient: 24 hours; Refrigerated: 5 days; Frozen: 4 months
The PPT must be received spun within 2 hours of draw to perform tests.

Alternative Specimen:

Lavender top- EDTA, White Top (PPT), Fluid-source required