Back to Test DirectoryTN96 : OnkoSight Advanced NGS BRAF Sequencing
INFORMATION:
Alternate Name:
OSA NGS BRAF
Methodology:
Genotyping by Next Generation Sequencing
Clinical Utility:
BRAF V600E/K mutation analysis is useful in the context of melanoma, colorectal cancer, thyroid cancer and hairy cell leukemia. Melanoma patients with V600E/K mutations are eligible for treatment with TKI inhibitor therapy. In colorectal cancer, BRAF may be used as a screening assay for MSI-H or unstable patients suspected of Lynch Syndrome. BRAF V600E can aid in the diagnosis of papillary thyroid cancer (PTC) from cytology samples. Numerous studies have shown BRAF to be associated with aggressive clinicopathologic features of PTC. BRAF can also be used to confirm a diagnosis of Hairy Cell Leukemia.
Gene:
BRAF
ORDERING:
Test Code:
TN96-8
Turnaround Time:
10 DAYS
Preferred Specimen:
Formalin-fixed, Paraffin-embedded Tissue
Collection:
| Container | Qty | Temp | Stability |
|---|---|---|---|
| Formalin-fixed, Paraffin-embedded Tissue | 1 | Room Temp |
Collection Instructions:
BLK: This comes in block form from client with surgical number imprint.
Storage Transport Instructions:
If submitting Bone Marrow or Peripheral Blood, 5 ml is required. Alternative specimen: Extracted DNA is acceptable, providing that the isolation of nucleic acid occurs in a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by CMS and/or the CAP.
Storage/Transport Instruction: Specimen can be sent either RT or refrigerated.
BLK: This comes in block form from client with surgical number imprint.
Custom Instruction: 10-15 unstained slides at 5 microns (tumor content>10%) with H&E, Shavings (if tumor content >40%) with H&E, stable for 30 days.
Specimen can be sent either room temperature or refrigerated. Ship with cold pack during warm weather.
Storage/Transport Instruction: Specimen can be sent either RT or refrigerated.
BLK: This comes in block form from client with surgical number imprint.
Custom Instruction: 10-15 unstained slides at 5 microns (tumor content>10%) with H&E, Shavings (if tumor content >40%) with H&E, stable for 30 days.
Specimen can be sent either room temperature or refrigerated. Ship with cold pack during warm weather.
Alternative Specimen:
Bone Marrow - Green Top, Peripheral Blood - Lavender Top, Peripheral Blood - Green Top, Bone Marrow - Lavender Top, Eppendorf tube
Billing:
CPT Codes:
81210 x 1
CPT Code Disclaimer
CPT codes provided are for informational purposes only. Accuracy of CPT presented should be validated prior to consideration for billing.
CPT coding is the sole responsibility of the billing party. Please direct any questions regarding CPT coding to the payer being billed.
